Composite

Part:BBa_K2926088

Designed by: Johanna Opgenoorth   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-21)


Basic Insert to express Cas13a (Lsh) and a short guideRNA array in S. cerevisiae

This part enables the expression of Cas13a (Lsh) and a guide RNA array containing three guide RNAs that target essential genes in S. cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5227
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5252
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1016
    Illegal BglII site found at 1562
    Illegal BglII site found at 1640
    Illegal BglII site found at 2327
    Illegal BglII site found at 3215
    Illegal BglII site found at 4205
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5227
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5227
    Illegal AgeI site found at 55
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 783
    Illegal SapI.rc site found at 1108
    Illegal SapI.rc site found at 1978
    Illegal SapI.rc site found at 2812


[edit]
Categories
Parameters
None