Composite

Part:BBa_K2926070

Designed by: Johanna Opgenoorth   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-21)


Basic insert coding for Cas13a(Lbu) to express it in S. cerevisiae

This part enables the expression of Cas13a (Lbu) in S. cerevisiae due to yeast-specific promoters and terminators.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4539
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4564
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 788
    Illegal BglII site found at 1724
    Illegal BglII site found at 1988
    Illegal BglII site found at 2492
    Illegal BglII site found at 2978
    Illegal BglII site found at 3086
    Illegal BglII site found at 3416
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4539
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4539
    Illegal AgeI site found at 55
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3097


[edit]
Categories
Parameters
None