Coding
Part:BBa_K2926068:Design
Designed by: Johanna Opgenoorth Group: iGEM19_Bielefeld-CeBiTec (2019-10-15)
Proline-glycine-peptide fused to the n-terminus of mCherry
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To enable the interaction of the n-terminal proline with its target transporter we designed a short sequence of prolines and glycines to minimize sterical hindrance and formation of secondary structures within the n-terminus.
Source
The proline-peptide was fused via add on PCR to the iGEM part BBa_J06504.