Coding
Part:BBa_K2926053:Design
Designed by: Johanna Opgenoorth Group: iGEM19_Bielefeld-CeBiTec (2019-10-15)
fluorescence reporter mCherry fused to pVIII from M13 bacteriophage with purification tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
By designing the sequence we added a short glycine serine linker between mCherry and pVIII to enable correct protein folding of both subdomains.
Source
Fusion protein from the iGEM part BBa_J06504 and the M13 bacteriophage protein pVIII from a helperphage plasmid obtained from NEB.