Coding

Part:BBa_K2926053:Design

Designed by: Johanna Opgenoorth   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-15)


fluorescence reporter mCherry fused to pVIII from M13 bacteriophage with purification tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

By designing the sequence we added a short glycine serine linker between mCherry and pVIII to enable correct protein folding of both subdomains.


Source

Fusion protein from the iGEM part BBa_J06504 and the M13 bacteriophage protein pVIII from a helperphage plasmid obtained from NEB.

References