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RBS

Part:BBa_K2924045

Designed by: Melanie Sbielut   Group: iGEM19_Duesseldorf   (2019-10-15)


RBS for bacillus subtilis

RBS for protein translation in B. subtilis


Usage and Biology

The RBS is the location in which a ribosome binds to the mRNA and initiates the translation. The following RBS can be used in Bacillus subtilis for translation of mRNA into protein. In our case the RBS was used to initiate the transcription of heterologous expressed casein and whey proteins in Bacillus subtilis (BBa_K2924033, BBa_K2924052, BBa_K2924054).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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