Composite

Part:BBa_K2924042:Design

Designed by: Andreas Nakielski   Group: iGEM19_Duesseldorf   (2019-10-16)


AOX promoter + Lactoferrin + HisTag + AOX Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BamHI site found at 2232
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1220
    Illegal BsaI.rc site found at 2215


Design Notes

Expression system for the bovine lactoferrin enzyme in Pichia pastoris

Source

The lactoferrin gene was cloned into the Pichia pastoris easy expression kit (Invitrogen)

References