Composite

Part:BBa_K2924017:Design

Designed by: Mirko Kraus   Group: iGEM19_Duesseldorf   (2019-10-16)


Promoter aldA with the reporter gene eYFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

This promoter is sensitive to long chain fatty acids and can be used to quantify them.

Source

The DNA sequence was take from Han et al.1 and synthesized by IDT.

References

1: Mee-Jung Han, Jeong Wook Lee, Sang Yup Lee, and Jong Shin Yoo. “Proteome-Level Responses of Escherichia coli to Long-Chain Fatty Acids and Use of Fatty Acid Inducible Promoter in Protein Production” Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Volume 2008, Article ID 735101,12 pages