RNA

Part:BBa_K2923016:Design

Designed by: Kateryna LEN   Group: iGEM19_Strasbourg   (2019-10-13)


Two PP7 stem-loops


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence contain AflII restrictions site : cttaag


Source

Pseudomonas phage PP7 [Berry, K.E., and Hochschild, A. (2018). A bacterial three-hybrid assay detects Escherichia coli Hfq-sRNA interactions in vivo. Nucleic Acids Research 46]

References

Berry, K.E., and Hochschild, A. (2018). A bacterial three-hybrid assay detects Escherichia coli Hfq-sRNA interactions in vivo. Nucleic Acids Research 46