Part:BBa_K2923010

LexA mutated repressor

LexA (22.7 kDa) is a transcriptional repressor involved in SOS-system (a DNA damage response) by its binding with a promoter sequence of SOS-response regulatory genes. This LexA repressor sequence was previously described in Dimitrova et al. 1998 as part of the new LexA-based genetic system for monitoring and analyzing protein heterodimerization. The detection process involves two fusions proteins, which are two versions of chimeric LexA dimer. This sequence corresponds to mutated LexA repressor, recognizing LexA mutated operator sequence CCGT.

Figure: Overview of LexA heterodimer repress system. The figure is reproduced from [Daines, D. A., Granger-Schnarr, M., Dimitrova, M., & Silver, R. P. (2002). Use of LexA-based system to identify protein-protein interactions in vivo. Methods in Enzymology, 153–161.doi:10.1016/s0076-6879(02)58087-3]

Usage and Biology

This mutated LexA corresponds to the selected sequence recognizing mutated LexA operator (WT sequence coming from E. coli strain JL1434) [Dimitrova, M., Younès-Cauet, G., Oertel-Buchheit, P., Porte, D., Schnarr, M., and Granger-Schnarr, M. (1998). A new LexA-based genetic system for monitoring and analyzing protein heterodimerization in Escherichia coli. Molecular and General Genetics MGG 257, 205–212.]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]