Part:BBa_K2922039:Design

The colicin-E1 operon under pBAD (Arabinose promoter) control with an amajLime chromoprotein reporte

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 125
  Illegal NheI site found at 2360
  Illegal NheI site found at 2383
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 65
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 642
  Illegal AgeI site found at 2000
  Illegal AgeI site found at 2057
  Illegal AgeI site found at 2189
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Each CDS are added an RBS (BBa_B0034) behind, and contain a terminator(BBa_B0010)in the end of colicin-E1 operon.

Source

This part constructs from BBa_K206000,BBa_K2922024,BBa_K2922025,BBa_K2922026,BBa_K1033914 by standard assembly..