Composite

Part:BBa_K2922012:Design

Designed by: Jisheng Xie   Group: iGEM19_XMU-China   (2019-10-13)


T7-RBS-bgl1A (Beta-D-glucosidase) functions in Kil secretion cassette with promoter J23109


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1507
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Construct from existed parts according to Standard BioBrick Assembly.


Source

Kil is from Escherichia coli and bgl1A comes from Saccharophagus degradans 2-40.

References