Composite

Part:BBa_K2922008:Design

Designed by: Tianyu Feng   Group: iGEM19_XMU-China   (2019-10-13)


Expression system of YebF-Cex coding YebF-Exoglucanase with T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 940
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 573
    Illegal NgoMIV site found at 946
    Illegal NgoMIV site found at 1448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 993
    Illegal SapI.rc site found at 1076


Design Notes

Construct from existed parts according to Standard BioBrick Assembly.


Source

YebF comes from Escherichia coli (Strain K12) and cex is from Cellulomonas fimi.

References