Composite
Part:BBa_K292007
Designed by: David Charoy Group: iGEM09_SupBiotech-Paris (2009-10-19)
CMV + GFP + Double terminator
This part contains a green fluorescence protein (GFP) under control of a CMV promoter and its double terminator (BBa_I712669 + BBa_B0014).
This bio-brick already contains a terminator, so it is ready to use.
Usage and Biology
This part is reliable to use when we want to know if a gene is inserted into a eukaryotic cell. For example we can use it when we design a recombinant virus. In fact we inserted this bio-brick into the synthetic virus; finally we are able to see if the virus can infect the targeted cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| biology | -NA- |
| direction | Bidirectional |
| emission | Green |
| excitation | -NA- |
| forward_efficiency | 0.604[CC]/0.96[JK] |
| protein | -NA- |
| reversed_version | 2145 |
| reverse_efficiency | 0.86[JK] |
| tag | None |
[edit]
Categories
Parameters
//classic/reporter/ret
//plasmid/measurement
//plasmid/measurement
| biology | |
| direction | Bidirectional |
| emission | Green |
| excitation | |
| forward_efficiency | 0.604[CC]/0.96[JK] |
| protein | |
| reversed_version | 2145 |
| reverse_efficiency | 0.86[JK] |
| tag | None |