Part:BBa_K292005

LacI repressor and a double terminator

This part contains a LacI repressor and a terminator (BBa_C0012 + BBa_B0014).

History:

In E.Coli, pLac is a part of the lactose operon. LacI is constantly expressed and represses the pLac. In the presence of Lactose, LacI is inhibited and pLac is active and allows the transcription of beta-galactosidase. Lactose can be substituted by IPTG (Isopropyl β-D-1-thiogalactopyranoside).

Usage and Biology

The LacI repressor repressed pLac promoter from the E.coli lactose operon. So LacI repressor is really reliable to use when we want to control a gene expression by using pLac promoter. In fact when LacI is not expressed, pLac is activated, and when there is an expression of LacI, pLac is inhibited. We can easily design a system to induce the transcription of LacI and creates a failed feedback mechanism for pLac inhibition.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1147
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Functional Parameters

N/A

biology	T7TE-LuxIA
direction	Bidirectional
forward_efficiency	0.604[CC]/0.96[JK]
function	Rep
ligands	allolactose, IPTG
protein	LacI
reversed_version	2145
reverse_efficiency	0.86[JK]
swisspro	P03023
tag	LVA