Part:BBa_K2916019:Design

Expression of GlnRS in E.coli

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1808
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 148
  Illegal BglII site found at 1096
  Illegal XhoI site found at 1757
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1702
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The protein was expressed in pET21a in BL21(DE3) E.coli strain.

Source

Sebastian Maerkl Lab at EPFL, Switzerland.

http://www.addgene.org/124108/

References

McClain, William H. “Rules That Govern TRNA Identity in Protein Synthesis.” Journal of Molecular Biology, vol. 234, no. 2, Nov. 1993, pp. 257–80. DOI.org (Crossref), doi:10.1006/jmbi.1993.1582.

Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568

Lavickova, Barbora, and Sebastian J. Maerkl. A Simple, Robust, and Low-Cost Method to Produce the PURE Cell - Free System. preprint, Synthetic Biology, 18 Sept. 2018. DOI.org (Crossref), doi:10.1101/420570.