Coding

Part:BBa_K2915350

Designed by: BENHAMMOU Yanis   Group: iGEM19_Aix-Marseille   (2019-10-10)


HhaI-methylase-CBD 6-His-Tag TEV site

HhaI is a methylase which is part of an enzymes groupes in part of transferase familly, and tranfert a donor methyl groupe into a an acceptor. This enzyme in our project can allows the binding of the " Rpob and KatG genes " meaning a TB gene into the cellulose membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 470
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick is in RFC 10 standards with:

The prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC  and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'

This HhaI-methylase exists with:

- with a His-tag and a TEV site

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Categories
Parameters
None