Part:BBa_K2915225:Design
IPTG inducible promoter (T7) with RBS TAL1 6-His-Tag with TEV site
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We add a 6Hig-Tag and a TeV cleavage site into the "TAL1" sequence which allows us to purify our proteins. We optimized the codons to be able to produce these proteins into the K12 E.coli strain. To perform the production of TAL1 in a K12 E.coli strain, we added a T7 promotor and a RBS into the psb1c3 plasmide,which is the regulator.. This biobrick is in RFC 10 standards with: The prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'
Source
Nucleotide sequence
References
Cermak T, et al. Efficient design and assembly of custom TALEs and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res 2011 Sep 1;39(17):7879.