DNA

Part:BBa_K2912010

Designed by: Lingling Liao   Group: iGEM19_SZU-China   (2019-10-13)


M. micrantha_leaves_ Unigene0029127

This is a single-stranded DNA that can be cyclized by T7 promoter since it has two sites complementary to T7 promoter. It can transcribe RNA interference (RNAi) molecule by rolling circle transcription, which can silence the gene encoding chlorophyll A-B binding protein CAB40 of M. micrantha（Unigene0029127）, through which we can block this essential metabolic gene expression to kill such invasive weed(illustrated in Fig. 1）.

Fig.1 The Relative Target Gene Expression（Unigene0029127）after Using hairpin siRNA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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