Part:BBa_K2912000:Design
RebA may act as a scaffolding protein to facilitate the major polymerization process
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is the first ORF of R-body, designated Reb A, extends from base 110 through 454. Reb A is preceded by the potential ribosome binding site (RBS) AGGAG, located 6 bp before the initiation codon. In E. coli, the consensus promoter sequences for the -10 and -35 regions are TTATA and TTGACA, respectively. The Reb A gene uses the TAA stop codon. Termination appears to be rho dependent since there are no strong inverted repeats followed by runs of thymidine.
Source
This part called rebA, belonging to the complete cds for the R body locus (reb) from Caedibacter taeniospiralis. The sequence is from the Genebank, and the protein ID is AAA73408.1.
References
[1] Pond FR, Gibson I, Lalucat J, et al. R-body-producing Bacteria.[J]. Microbiol Rev, 1989, 53(1): 25-67.
[2] Matsuoka JI, Ishizuna F, Kurumisawa K, et al. Stringent Expression Control of Pathogenic R-body Production in Legume Symbiontazorhizobium Caulinodans[J]. Mbio, 2017, 8(4): 0-17.
[3] Heruth DP, Pond FR, Dilts JA, et al. Characterization of Genetic Determinants for R Body Synthesis and Assembly in Caedibacter Taeniospiralis 47 and 116.[J]. Journal of Bacteriology, 1994, 176(12): 3559-3567.
[4] Quackenbush RL, Burbach JA. Cloning and Expression of Dna Sequences Associated with the Killer Trait of Paramecium Tetraurelia Stock 47[J]. Proceedings of the National Academy of Sciences of the United States of America, 1983, 80(1): 250-254.