Plasmid

Part:BBa_K2911001

Designed by: Ryan Lambert   Group: iGEM19_USAFA   (2019-10-14)


Empty pSRKBB plasmid

Rhodoccocus Plasmid pSRKBB. This plasmid consists can be used in both E. coli and Rhodoccocus. This plasmid has multiple cut sites including; SpeI, EcoRI, PstI. Origin of replication pSKBB-empty [4566-5141]. Antibiotic resistant gene NeoR/KanR. Used previously by Edinburgh Overgraduate iGEM team.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2049
    Illegal XbaI site found at 2067
    Illegal SpeI site found at 2356
    Illegal PstI site found at 2374
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2049
    Illegal SpeI site found at 2356
    Illegal PstI site found at 2374
    Illegal NotI site found at 2282
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2049
    Illegal BglII site found at 2206
    Illegal BamHI site found at 2212
    Illegal XhoI site found at 2290
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2049
    Illegal XbaI site found at 2067
    Illegal SpeI site found at 2356
    Illegal PstI site found at 2374
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2049
    Illegal XbaI site found at 2067
    Illegal SpeI site found at 2356
    Illegal PstI site found at 2374
    Illegal NgoMIV site found at 748
    Illegal NgoMIV site found at 1031
    Illegal NgoMIV site found at 4209
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5369
    Illegal SapI.rc site found at 597
    Illegal SapI.rc site found at 807
    Illegal SapI.rc site found at 1964


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