Measurement
Part:BBa_K2909007:Design
Designed by: Laurent CHEN Group: iGEM19_Sorbonne_U_Paris (2019-08-27)
ParoR_HiBiT-CloverGFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal PstI site found at 2846 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal NheI site found at 269
Illegal PstI site found at 2846
Illegal NotI site found at 1779 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal BamHI site found at 11
Illegal BamHI site found at 3453
Illegal XhoI site found at 2320 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal PstI site found at 2846 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal PstI site found at 2846 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 3491
Design Notes
Source
All parts comes from the Chlamydomonas reinhardtii MoClo Kit (Crozet et al. 2018).
References
- Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2074–2086 (2018).
- 1.Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS ONE 6, e16765 (2011).