DNA
Part:BBa_K2908676:Design
Designed by: Dong Yufan Group: iGEM19_CSU_CHINA (2019-10-16)
s(GATA3)p-GAD-miR101-BS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 99
Illegal NheI site found at 584 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 360
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 279
Design Notes
The initiation codon ATG can only appear between miRNA sponse and EGFP.
Source
We designed it by ourselves including improving the BBa_K2580666 to enhance its specificity and synthesized it by company.