Part:BBa_K2908667

yCD

Figure1.This figure shows the crystal structure of yeast cytosine deaminase (yCD).

The cytosine deaminase (CD)/5-fluorocytosine (5-FC) approach is the next most widely studied suicide gene therapy approach. CD is uniquely expressed in certain fungi and bacteria and it converts the prodrug 5-FC (used to treat infections by fungi such as Candida albicans and Cryptococcus neoformans) into the active agent 5-fluorouracil (5-FU). While 5-FC is nontoxic to human cells because of the lack of CD, 5-FU is used to treat cancers like colon, pancreatic, and breast cancer. The cytotoxic effects of 5-FU occur following its conversion to 5-fluoro-2'-deoxyuridine-5'-monophosphate (5-FdUMP). 5-FdUMP is an irreversible inhibitor of thymidylate synthase and thus inhibits DNA synthesis by deoxythymidine triphosphate (dTTP) deprivation and causes DNA strand breakage, leading to cell death.
We add yCD to our gene circuit to trigger the death of the triple-negative breast cancer.(TNBC)

Figure2. (A)This presented picture shows that after 2days adding the 5-FC, yCD in our circuit play a role in killing the cancer cells-MDA-MB-231.
(B) This figure shows the same results using another method-DAPI staining, to show the morphology of nucleus.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 854
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 466
  Illegal BsaI.rc site found at 503