Composite

Part:BBa_K2905018:Design

Designed by: David Viguilla   Group: iGEM19_Alma   (2019-10-16)


TAG-Read through reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1525
    Illegal AgeI site found at 1637
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An inducible promoter was used to help control expression.


Source

DNA sequences obtained from the registry, synthesized by Twist Biosciences

References