Reporter

Part:BBa_K2905016:Design

Designed by: David Viguilla   Group: iGEM19_Alma   (2019-10-15)


High pH Sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1661
    Illegal AgeI site found at 1773
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 332


Design Notes

To fit size requirements from Twist, some scar sequences were condensed to 2-bps


Source

Twist Bioscience

References