Coding

Part:BBa_K2905012

Designed by: Brianna Ricker   Group: iGEM19_Alma   (2019-06-24)


PylS

Pyrrolysine--tRNA ligase. Starts with a His-6 tag, followed by a NheI site scar and a TEV protease recognition site, fused in frame to the PylS gene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 43
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None