Device

Part:BBa_K2904054:Design

Designed by: Peng Deng   Group: iGEM19_OUC-China   (2019-10-15)


aTc inducible sfGFP regulated by modular Adda riboswitch containing Tuner E


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 425


Design Notes

This part was used to validate our design principle.


Source

Science Museum No.105，College of Marine Life Sciences, Ocean University of China

References

Folliard T , Mertins B , Steel H , et al. Ribo-attenuators: novel elements for reliable and modular riboswitch engineering[J]. Scientific Reports, 2017, 7(1):4599.