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Reporter
Part:BBa_K2890006:Design
Designed by: Jin-Shu Yang Group: iGEM18_HFLS_ZhejiangUnited (2018-10-07)
HxlR + eRFP + FDM
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1479
Illegal NheI site found at 1502 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2454
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1176
Illegal AgeI site found at 1288
Illegal AgeI site found at 1741
Illegal AgeI site found at 2053 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1585
Design Notes
There are two strategies for formaldehyde detection (HxlR and frmR), and also two strategies for formaldehyde degradation (PADH+FDH and FDM). Therefore, there are four possible choices for the combinations. Here is one of four possibilities.
Source
Combination of HxlR+eRFP (BBa_K2890004) and FDM (BBa_K2890003).