Reporter

Part:BBa_K2890006:Design

Designed by: Jin-Shu Yang   Group: iGEM18_HFLS_ZhejiangUnited   (2018-10-07)


HxlR + eRFP + FDM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1479
    Illegal NheI site found at 1502
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2454
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1176
    Illegal AgeI site found at 1288
    Illegal AgeI site found at 1741
    Illegal AgeI site found at 2053
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1585


Design Notes

There are two strategies for formaldehyde detection (HxlR and frmR), and also two strategies for formaldehyde degradation (PADH+FDH and FDM). Therefore, there are four possible choices for the combinations. Here is one of four possibilities.


Source

Combination of HxlR+eRFP (BBa_K2890004) and FDM (BBa_K2890003).

References