Composite

Part:BBa_K2888010:Design

Designed by: SBS_SH_112144   Group: iGEM18_SBS_SH_112144   (2018-10-07)


fused mlrA gene with histag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 770
    Illegal AgeI site found at 305
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We add 6x HIS to the existed DNA sequence

Source

This mlrA gene is a part of the microcystin degradation system discovered in Sphingopyxis sp. C-1 by Shimuzu.

References