Part:BBa_K2888003:Design

fused lysozyme gene with 6x His tag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We add 6x histag to the whole sequence via PCR

Source

This cyanophage lysozyme is discovered by Heideburg et al in Yellowstone National Park {Heideburg:2009hb}