Coding

Part:BBa_K2886008:Design

Designed by: Leijie Dai   Group: iGEM18_Fudan-CHINA   (2018-09-27)


VEGF-scFv-LEUCINE_ZIPPER-cECFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 524
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is used as a positive control of our experiment because it could emit fluorescence. even without the ligand VEGF. And it should used with BBa_K2886009 The actual sequence of this part is different from the sequence page.Delete the scar and inset the sequence ggcgccgggacaggagggtctgggggtggcactggcggatctggaggagactacaaggacgatgacgacaacggcggatcgggggggtctggtggtagtggcggttcggggggaggtaccggtctggtcgtggttgctggagtcctgttttgttatggcttgctagtgacagtggctctttgtgttggaggcggaggctcc and ggaggcggaggctcc between BBa_K2886002 & BBa_K2886006 and BBa_K2886006 & BBa_K2886004 respectively.


Source

It is a composite part made from BBa_K2886002, BBa_K2886006 and BBa_K2886004.

References