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Composite
Part:BBa_K2878006:Design
Designed by: Yilin Mo Group: iGEM18_SSHS-Shenzhen (2018-09-30)
ARK-shRNA-1 template with T7 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
ARK-shRNA-1 template sequence starts with GG, which is for transcription efficiency. GC content is around 50%, its target site is not in the first 75 bases after AUG, these are for the RNAi efficiency. UU overhangs in 3′-end, this is for the stability of shRNA. The antisense strand of shRNA is reverse complement with the sequence of the target site.
Source
ARK-shRNA-1 template is designed from the sequence of the target site in Arginine Kinase mRNA of Phyllotreta striolata, which is 997-1018 after AUG. shRNA contains a sense strand, a loop, and an antisense strand.