RNA
Part:BBa_K2877024
Designed by: Andrew D Ellington Group: iGEM18_Austin_LASA (2018-10-11)
Rev crRNA 4 (AsCas12a)
This is a crRNA sequence for Rev. It includes a hairpin sequence for AsCas12a and as such should be used with AsCas12a. Following the hairpin sequence is a spacer sequence from Rev that serves as the main method in which Cas12a identifies our viral DNA. These sequences can either be ordered as RNA oligos or transcribed via T7 in vitro transcription by adding a T7 promoter at the start of the sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |