Part:BBa_K2876006
alpha+linker
This part fused the alpha subunit of a RNAPolIII,BBa_K2876002, (https://parts.igem.org/Part:BBa_K2876002) with a tri-alanine linker for fusion to other proteins. ]
Inspired by the yeast two-hybrid system designed iGEM Tsinghua 2017 [3], we fused antibodies and dCas9 proteins to the subunits of the P2H system, creating a versatile platform capable of detecting specific DNA sequences, small molecules, and proteins. Our DNA detection system works with dCas9-protein fusions, which can be targeted to specific DNA sequences (FIGURE 2: DNA detection figure), and is called casP2H (cas9 prokaryotic two hybrid system). Our protein and small molecule system works by fusing single-chain-antibodies specific to different epitopes of a target protein or small molecule, and is called SCAP2H (single chain antibody prokaryotic two hybrid system).
This detection system is unique because it utilizes transcription. Detection-induced transcription allows genes to be activated by the presence of a user-selected molecule, therefore creating a genetic output to a chosen input. For example, a therapeutic protein could be transcribed in response to a disease biomarker.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 8
Illegal NotI site found at 774 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 480
- 1000COMPATIBLE WITH RFC[1000]
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