Regulatory
Part:BBa_K2876000:Experience
Designed by: Isaac Justice Group: iGEM18_Stanford (2018-06-22)
To test the reporter we transformed DH5-alpha with the pOL2-62 + RFP reporter plasmid, as well as the positive control bacteriomatch lambda and alpha plasmids from Addgene (https://www.addgene.org/browse/article/8393/) and induced with IPTG. Unfortunately, when we ran the experiment on the plate reader we saw no fluorescence response (Figure 1).
Figure 1: 48hr plate reader fluorescence experiment of pOL2-62 + RFP reporter with bacteriomatch system (positive control lambda and alpha fusion proteins) and induced with IPTG.
Applications of BBa_K2876000
prokaryotic two hybrid systems
User Reviews
UNIQ02816f67fea2aea5-partinfo-00000000-QINU UNIQ02816f67fea2aea5-partinfo-00000001-QINU