![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K2873003:Design
Designed by: Zhipeng Ding Group: iGEM18_Minnesota (2018-10-10)
LacI-glnA-GFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1375
Illegal EcoRI site found at 1645
Illegal PstI site found at 2666 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1375
Illegal EcoRI site found at 1645
Illegal PstI site found at 2666 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1375
Illegal EcoRI site found at 1645
Illegal BglII site found at 1961
Illegal BamHI site found at 2337 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1375
Illegal EcoRI site found at 1645
Illegal PstI site found at 2666 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1375
Illegal EcoRI site found at 1645
Illegal PstI site found at 2666
Illegal AgeI site found at 1803
Illegal AgeI site found at 2238 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3329
Design Notes
LacI-glnA-GFP)is constructed from pSB1C3 plasmid backbone consisting LacI protein coding region, LacI binding site, glnA coding region and GFP. LacI protein coding region overexpression of LacI to ensure inhibition of LacI promoter LacI binding site: controlled promoter region that only operates when LacI is released from IPTG binding glnA coding region: expression of glutamine synthetase GFP: Green fluorescent protein marker for characterizing the expression level of glnA
Source
Gibson assembly product sourced from existing plasmids in Zhang lab.