Device

Part:BBa_K2873003

Designed by: Zhipeng Ding   Group: iGEM18_Minnesota   (2018-10-10)


LacI-glnA-GFP

This part is IPTG controlled auxotrophic plasmid used in strain JW3841-41

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1375
    Illegal EcoRI site found at 1645
    Illegal PstI site found at 2666
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1375
    Illegal EcoRI site found at 1645
    Illegal PstI site found at 2666
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1375
    Illegal EcoRI site found at 1645
    Illegal BglII site found at 1961
    Illegal BamHI site found at 2337
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1375
    Illegal EcoRI site found at 1645
    Illegal PstI site found at 2666
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1375
    Illegal EcoRI site found at 1645
    Illegal PstI site found at 2666
    Illegal AgeI site found at 1803
    Illegal AgeI site found at 2238
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3329


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