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Part:BBa_K2873002:Design
mer-glnA-GFP(RBS)
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 5752
Illegal EcoRI site found at 3724
Illegal EcoRI site found at 3994
Illegal PstI site found at 3581
Illegal PstI site found at 5015 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 3724
Illegal EcoRI site found at 3994
Illegal NheI site found at 573
Illegal SpeI site found at 5753
Illegal PstI site found at 3581
Illegal PstI site found at 5015
Illegal PstI site found at 5767
Illegal NotI site found at 7
Illegal NotI site found at 5760 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 3724
Illegal EcoRI site found at 3994
Illegal BglII site found at 4310
Illegal BamHI site found at 4686 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 5753
Illegal EcoRI site found at 3724
Illegal EcoRI site found at 3994
Illegal PstI site found at 3581
Illegal PstI site found at 5015 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 3724
Illegal EcoRI site found at 3994
Illegal XbaI site found at 16
Illegal SpeI site found at 5753
Illegal PstI site found at 3581
Illegal PstI site found at 5015
Illegal PstI site found at 5767
Illegal NgoMIV site found at 2442
Illegal NgoMIV site found at 2490
Illegal NgoMIV site found at 2552
Illegal NgoMIV site found at 2763
Illegal NgoMIV site found at 3356
Illegal AgeI site found at 4152
Illegal AgeI site found at 4587 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 5678
Illegal SapI site found at 558
Design Notes
Contains a full-length Mer operon consists of MerR (mercuric regulatory protein), transporter protein MerP and MerT, Mercury reductase MerA, and followed by glnA and GFP coding sequence. The function of MerR is very similar to LacI, it binds to the promoter region when no ionic mercury is present and inhibits transcription. When ionic mercury is present, it binds to MerR followed by MerR release.
Functionally wise, glnA, GFP and merA will all be expressed when ionic mercury is present. glnA will support bacteria growth and merA reducing ionic mercury; when the environmental mercury has all been exhausted, glnA synthesis will stop due to the inhibition of transcription and bacteria will eventually die off.
Promoter region between mer operon and glnA is RBS
Source
BBa_K1420000