Device

Part:BBa_K2873002:Design

Designed by: Zhipeng Ding   Group: iGEM18_Minnesota   (2018-10-10)


mer-glnA-GFP(RBS)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 5752
    Illegal EcoRI site found at 3724
    Illegal EcoRI site found at 3994
    Illegal PstI site found at 3581
    Illegal PstI site found at 5015
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 3724
    Illegal EcoRI site found at 3994
    Illegal NheI site found at 573
    Illegal SpeI site found at 5753
    Illegal PstI site found at 3581
    Illegal PstI site found at 5015
    Illegal PstI site found at 5767
    Illegal NotI site found at 7
    Illegal NotI site found at 5760
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 3724
    Illegal EcoRI site found at 3994
    Illegal BglII site found at 4310
    Illegal BamHI site found at 4686
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 5753
    Illegal EcoRI site found at 3724
    Illegal EcoRI site found at 3994
    Illegal PstI site found at 3581
    Illegal PstI site found at 5015
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 3724
    Illegal EcoRI site found at 3994
    Illegal XbaI site found at 16
    Illegal SpeI site found at 5753
    Illegal PstI site found at 3581
    Illegal PstI site found at 5015
    Illegal PstI site found at 5767
    Illegal NgoMIV site found at 2442
    Illegal NgoMIV site found at 2490
    Illegal NgoMIV site found at 2552
    Illegal NgoMIV site found at 2763
    Illegal NgoMIV site found at 3356
    Illegal AgeI site found at 4152
    Illegal AgeI site found at 4587
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5678
    Illegal SapI site found at 558


Design Notes

Contains a full-length Mer operon consists of MerR (mercuric regulatory protein), transporter protein MerP and MerT, Mercury reductase MerA, and followed by glnA and GFP coding sequence. The function of MerR is very similar to LacI, it binds to the promoter region when no ionic mercury is present and inhibits transcription. When ionic mercury is present, it binds to MerR followed by MerR release.

Functionally wise, glnA, GFP and merA will all be expressed when ionic mercury is present. glnA will support bacteria growth and merA reducing ionic mercury; when the environmental mercury has all been exhausted, glnA synthesis will stop due to the inhibition of transcription and bacteria will eventually die off.

Promoter region between mer operon and glnA is RBS


Source

BBa_K1420000

References