Part:BBa_K2873000

mer-glnA-GFP(pdu1358)

contains a full length Mer operon, consisted by MerR(mercuric regulatory protein), transporter protein MerP and MerT, Mercury reductase MerA, followed by glnA and gfp coding sequence.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 1
  Illegal suffix found in sequence at 5849
  Illegal EcoRI site found at 3821
  Illegal EcoRI site found at 4091
  Illegal PstI site found at 5112
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1
  Illegal EcoRI site found at 3821
  Illegal EcoRI site found at 4091
  Illegal NheI site found at 573
  Illegal SpeI site found at 5850
  Illegal PstI site found at 5112
  Illegal PstI site found at 5864
  Illegal NotI site found at 7
  Illegal NotI site found at 5857
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1
  Illegal EcoRI site found at 3821
  Illegal EcoRI site found at 4091
  Illegal BglII site found at 4407
  Illegal BamHI site found at 4783
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 1
  Illegal suffix found in sequence at 5850
  Illegal EcoRI site found at 3821
  Illegal EcoRI site found at 4091
  Illegal PstI site found at 5112
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 1
  Illegal EcoRI site found at 3821
  Illegal EcoRI site found at 4091
  Illegal XbaI site found at 16
  Illegal SpeI site found at 5850
  Illegal PstI site found at 5112
  Illegal PstI site found at 5864
  Illegal NgoMIV site found at 2442
  Illegal NgoMIV site found at 2490
  Illegal NgoMIV site found at 2552
  Illegal NgoMIV site found at 2763
  Illegal NgoMIV site found at 3356
  Illegal AgeI site found at 4249
  Illegal AgeI site found at 4684
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 5775
  Illegal SapI site found at 558