Composite

Part:BBa_K2871006:Design

Designed by: Nattawat Leelahakorn   Group: iGEM18_UCopenhagen   (2018-10-08)


Cassette containing CesF and CesT T3SS substrate chaperones


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 40
    Illegal BglII site found at 545
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 71


Design Notes

Both CesF (BBa_K2871003) and CesT (BBa_K2871004) CDS are preceded by weak-medium strength RBS, BBa_B0032. 6X Histidine tag is added to the C-terminal of each chaperone gene to facilitate expression-tracing in western blot.

Source

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References