Reporter

Part:BBa_K2871002

Designed by: Nuttawat Leelahakorn   Group: iGEM18_UCopenhagen   (2018-09-24)


mCherry, N-terminally fused with Map20 signal for T3SS export

The mCherry fluorescent protein from BBa_J06504 fused with the polypeptide signal sequence map20 (BBa_K2871000), at the N-terminal. This enables mCherry to be translocated out of the cell via the bacterial Type-3-secretion system (T3SS). the signal sequence is derived from the map gene from the Enteropathogenic E. coli strain E22.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None