Regulatory
MT2

Part:BBa_K2869001:Design

Designed by: Daniel Russell Graves   Group: iGEM18_UC_Davis   (2018-10-09)


inducible promoter from Metallothionein 2 (derived from HEK cell line)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We selected this promoter for submission to the registry as it is RFC10 compatible and is part of a transcriptionally regulated stress pathway. This part has been verified by sequencing.



Source

This part was amplified out of HEK genomic DNA.

References

Santos AK, Parreira RC, Resende RR. Expression System Based on an MTIIa Promoter to Produce hPSA in Mammalian Cell Cultures. Frontiers in Microbiology. 2016;7:1280. doi:10.3389/fmicb.2016.01280.