Composite

Part:BBa_K2868011:Design

Designed by: Cale Lester   Group: iGEM18_Stanford-Brown-RISD   (2018-08-01)


T7 histag-Chitin-Binding


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 69
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for production in E.Coli, and illegal iGEM cut sites have been removed.


Source

Chitin binding protein is from Bacillus circulans.

References