Regulatory
cadA promo

Part:BBa_K285101:Design

Designed by: Xing Xiong   Group: iGEM09_Cornell   (2009-08-10)

cadA regulatory region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CadA regulatory region increases expression in response to higher concentrations of certain heavy metals such as Cd, Zn, and Co. Among these metals CadA regulatory region is most responsive to Cadmium. The part requires the czrA regulon found in Bacillus Subtitles to function correctly.[1][2]

Source

Ahmed Gaballa & John D. Helmann Bacillus subtilis CPx-type ATPases: characterization of Cd, Zn, Co and Cu efflux systems. Biometals. 2003 Dec;16(4):497-505.

References

[1]Moore CM, Gaballa A, Hui M, Ye RW, Helmann JD. Genetic and physiological responses of Bacillus subtilis to metal ion stress. Mol Microbiol. 2005 Jul;57(1):27-40.

[2]Ahmed Gaballa & John D. Helmann Bacillus subtilis CPx-type ATPases: characterization of Cd, Zn, Co and Cu efflux systems. Biometals. 2003 Dec;16(4):497-505.