Other
Part:BBa_K2847000:Design
Designed by: Slavil Peykov Group: iGEM18_Bulgaria (2018-10-09)
Cer sequence from plasmid ColE1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Standard design
If you want to have this part synthesized by IDT, order the following sequence:
gRNA cloning cassette gaattcgcggccgcttctagagCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGA TTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATCGCGCAGTTTTTAGGGTGGTTTG TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCC TCGTAGCCATCGAGATAAACCGCAGAAATCGTGTCAGCCAGCAGCCTGGATTTTTTCGTtactagtagcggccgctgcag
PCR amplify it using the following primers:
C_BioBrick_Prefix-F: TATGAATTCGCGGCCGCTTCTAG
C_BioBrick_Suffix-R: TATCTGCAGCGGCCGCTACTAGTA
and then clone it into a pSB vector of choice.
Source
This part was obtained as gBlock fragment by IDT