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Part:BBa_K2843001

Designed by: Camille Lorie   Group: iGEM18_East_Chapel_Hill   (2018-10-07)


CHOPv2

This part is an improvement of the 2017 East Chapel Hill iGem "CHOP" (Chloramphenicol Acetyltranferase Operon). At fluoride concentrations of 2uM and above, the delta-CRCB E coli strain (strain JW0619-1 from the Yale Coli Genetics Stock Center) will grow on chloramphenicol. This vector is designed with Xhol cutting sites so one may easily remove and replace different segments of the operon to screen for combinations of riboswitch and promoter with highest effectivity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 837
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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