RNA
Part:BBa_K2841524
Designed by: Rhys Evans, Jack Lawrence, Jonny Whiteside Group: iGEM18_Warwick (2018-10-13)
Unfolding RNA hairpin loop Delta
This hairpin loop structure can be unfolded by the complementary base pairing of a target primary sequence of RNA, or "sense" RNA (sRNA). The sRNA can bind to regions VR1 and VR2, causing the unfolding of the hairpin loop as the clamp region is released from the 20bp region, opening up these regions for function (e.g opens up a ribosome binding site / targeting region of a gRNA).
Changing the VR1 and VR2 regions changes what RNA sequence will activate the part. Changing the clamp and 20bp regions will change the function of the unfolded RNA.
Our constructs are Alpha, Beta, Gamma, Delta, and Epsilon
Secondary structure prediction of hairpin RNA
Complemented by Part BBa_K2841534
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |