RNA

Part:BBa_K2841521:Design

Designed by: Rhys Evans, Jack Lawrence, Jonny Whiteside   Group: iGEM18_Warwick   (2018-10-13)


Unfolding RNA hairpin loop Alpha


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Changing the VR1 and VR2 regions changes what RNA sequence will activate the part. Changing the clamp and 20bp regions will change the function of the unfolded RNA.


Source

Rodrigo, G., Landrain, T.E. and Jaramillo, A. (2012) De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A, 109, 15271-15276.

References