RNA
Part:BBa_K2841515:Design
Designed by: Jack Lawrence, Rhys Evans, Jonny Whiteside Group: iGEM18_Warwick (2018-10-14)
MG1655 Specific EndA Mutant Recognition Device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 158
Illegal NgoMIV site found at 187 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The specific structure of the RNA device had to be precise otherwise the device would cease to function.
Source
DH5 Alpha EndA Mutant Information: https://microbewiki.kenyon.edu/index.php/DH5-Alpha_E.coli Genomic Information: https://www.ncbi.nlm.nih.gov/pubmed/18245285 MG1655 EndA Sequence Source: https://biocyc.org/ECOLI/sequence?type=GENE&object=EG11336