Device

Part:BBa_K2841500:Design

Designed by: Jack Lawrence, Rhys Evans, Jonny Whiteside   Group: iGEM18_Warwick   (2018-10-09)


Legionella Sensing LacI Repressing Alpha Toxicity Experimental Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1077
    Illegal NgoMIV site found at 1106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 856


Design Notes

Had to insert the gRNA into the vector


Source

Toxicity - https://aem.asm.org/content/81/7/2506

Base part - BBa_K741002

Other Details - BBa_K2841541

References